1GNQ
X-RAY CRYSTAL STRUCTURE ANALYSIS OF THE CATALYTIC DOMAIN OF THE ONCOGENE PRODUCT P21H-RAS COMPLEXED WITH CAGED GTP AND MANT DGPPNHP
Experimental procedure
Temperature [K] | 277 |
Detector technology | AREA DETECTOR |
Collection date | 1992 |
Detector | SIEMENS |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 40.800, 40.800, 158.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 * - 2.500 |
R-factor | 0.218 * |
Rwork | 0.207 |
R-free | 0.26900 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.347 * |
Data reduction software | XDS |
Phasing software | X-PLOR (3.0) |
Refinement software | X-PLOR (3.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.065 * |
Total number of observations | 14166 * |
Number of reflections | 4544 |
Completeness [%] | 71.6 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.6 * | used to seed * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | PEG400 | 25 (%(v/v)) | |
3 | 1 | reservoir | Tris-HCl | 64 (mM) | |
4 | 1 | reservoir | 10 (mM) | ||
5 | 1 | reservoir | DTT | 20 (mM) |