1GNP

X-RAY CRYSTAL STRUCTURE ANALYSIS OF THE CATALYTIC DOMAIN OF THE ONCOGENE PRODUCT P21H-RAS COMPLEXED WITH CAGED GTP AND MANT DGPPNHP

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1992
Detector	SIEMENS
Spacegroup name	P 32 2 1
Unit cell lengths	40.080, 40.080, 160.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 * - 2.700
R-factor	0.193
Rwork	0.193
RMSD bond length	0.008
RMSD bond angle	1.440 *
Data reduction software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.600
Rmerge	0.098 *
Total number of observations	15930 *
Number of reflections	5007
Completeness [%]	88.8
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG400	22 (%(v/v))
3	1	reservoir	Tris-HCl	64 (mM)
4	1	reservoir		10 (mM)
5	1	reservoir	DTE	5 (mM)