1GNO
HIV-1 PROTEASE (WILD TYPE) COMPLEXED WITH U89360E (INHIBITOR)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | P 61 |
| Unit cell lengths | 63.160, 63.160, 83.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.174 * |
| Rwork | 0.174 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.051 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | CCP4 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.039 |
| Completeness [%] | 90.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.3 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | inhibitor | 4.0 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | sodium phosphate | 10 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 34 (%) | |
| 5 | 1 | reservoir | dimethyl sulfoxide | 10 (%) | |
| 6 | 1 | reservoir | beta-mercaptoethanol | 30 (mM) | |
| 7 | 1 | reservoir | 2-propanol | 2 (%) | |
| 8 | 1 | reservoir | sodium phosphate/citric acid | 200 (mM) |
