1GNN
HIV-1 PROTEASE MUTANT WITH VAL 82 REPLACED BY ASN (V82N) COMPLEXED WITH U89360E (INHIBITOR)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | P 61 |
| Unit cell lengths | 63.260, 63.260, 83.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.183 * |
| Rwork | 0.183 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.053 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | CCP4 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.038 |
| Completeness [%] | 97.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | inhibitor | 5.0 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | sodium acetate | 20 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 38 (%) | |
| 5 | 1 | reservoir | dimethyl sulfoxide | 10 (%) | |
| 6 | 1 | reservoir | beta-mercaptoethanol | 30 (mM) | |
| 7 | 1 | reservoir | 2-propanol | 4 (%) | |
| 8 | 1 | reservoir | sodium phosphate/citric acid | 200 (mM) |
