1GNL
Hybrid Cluster Protein from Desulfovibrio desulfuricans X-ray structure at 1.25A resolution using synchrotron radiation at a wavelength of 0.933A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.330, 61.650, 72.250 |
| Unit cell angles | 82.77, 73.67, 87.31 |
Refinement procedure
| Resolution | 28.630 - 1.250 |
| R-factor | 0.13 |
| Rwork | 0.129 |
| R-free | 0.14500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB FROM ENTRY 1GN9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.564 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0.36) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.630 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.054 * | 0.215 * |
| Total number of observations | 915955 * | |
| Number of reflections | 247243 | |
| <I/σ(I)> | 8 | 3.1 |
| Completeness [%] | 94.9 | 85.3 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.6 * | 293 * | 25% PEG 4000, 0.1M MES PH6.0, T=277K, pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 25 (%) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
| 3 | 1 | drop | protein | 12.6 (mg/ml) | |
| 4 | 1 | drop | Tris-HCl | 20 (mM) | pH7.6 |
| 5 | 1 | drop | 0.2 (M) |
