1GNJ

HUMAN SERUM ALBUMIN COMPLEXED WITH cis-5,8,11,14-EICOSATETRAENOIC ACID (ARACHIDONIC ACID)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	298
Detector technology	CCD
Collection date	2000-07-15
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	190.219, 38.972, 96.236
Unit cell angles	90.00, 105.75, 90.00

Refinement procedure

Resolution	29.620 - 2.600
R-factor	0.194
Rwork	0.194
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bj5
RMSD bond length	0.006 *
RMSD bond angle	1.270 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.600	2.760
High resolution limit [Å]	2.600	2.600
Rmerge	0.078	0.311
Number of reflections	20374
<I/σ(I)>	5.4	2.3
Completeness [%]	94.9	76
Redundancy	5.3	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	4 *	pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	100 (mg/ml)
2	1	reservoir	PEG3350	36-37 (%(w/v))
3	1	reservoir	potassium phosphate	50 (mM)	pH7.5