1GML
crystal structure of the mouse CCT gamma apical domain (triclinic)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-03 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.740, 65.020, 65.470 |
| Unit cell angles | 89.97, 103.95, 90.35 |
Refinement procedure
| Resolution | 28.950 - 2.200 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e0r |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.430 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 * | 2.310 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.058 | 0.150 |
| Number of reflections | 39106 | |
| <I/σ(I)> | 8 | 4.2 |
| Completeness [%] | 93.0 | 92.6 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 14 * | CRYSTALS GROWN BY HANGING DROP METHOD USING A 1:1 MIXTURE OF 12MG/ML PROTEIN, 400MM NACL, 20% GLYCEROL, 8MM TRIS PH8.0, 0.4MM EDTA AND 100MM TRIS PH8.0, 200MM NACL, 10MM MG(OAC)2, pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH8.0 |
| 3 | 1 | drop | 300 (mM) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | EDTA | 0.2 (mM) | |
| 6 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 7 | 1 | reservoir | Tris | 100 (mM) | pH8.0 |
| 8 | 1 | reservoir | 200 (mM) | ||
| 9 | 1 | reservoir | 10 (mM) |
