1GMD

X-ray crystal structure of gamma-chymotrypsin in hexane

Experimental procedure

Spacegroup name	P 42 21 2
Unit cell lengths	69.340, 69.320, 97.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 * - 2.200
R-factor	0.176
Rwork	0.176
RMSD bond length	0.012
RMSD bond angle	1.820
Refinement software	X-PLOR (1GMD)

Data quality characteristics

	Overall
High resolution limit [Å]	2.200
Rmerge	0.092 *
Total number of observations	13982 *
Number of reflections	7606 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	5.6 *		referred to 'Stoddard, B. L.', (1990) Biochemistry, 29, 4871-4879 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	chymotrypsin	15 (mg/ml)
2	1	1	sodium cacodylate	10 (mM)
3	1	1	cetyltrimethylammonium bromide	0.75 (%sat)
4	1	1	ammonium sulfate	45 (%sat)
5	1	2	ammonium sulphate	65 (%sat)