1GM5
Structure of RecG bound to three-way DNA junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.704, 144.602, 84.023 |
| Unit cell angles | 90.00, 113.82, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.240 |
| R-factor | 0.275 |
| Rwork | 0.272 |
| R-free | 0.32800 |
| Structure solution method | MIR |
| RMSD bond length | 0.021 * |
| RMSD bond angle | 2.600 * |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.250 |
| Rmerge | 0.039 |
| Number of reflections | 22945 |
| Completeness [%] | 98.3 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | pH 5.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | Tris-HCl | 10 (mM) | |
| 5 | 1 | reservoir | potassium phosphate | 500 (mM) | |
| 6 | 1 | reservoir | 5 (mM) | ||
| 7 | 1 | reservoir | ADP | 1 (mM) |
