1GLU
CRYSTALLOGRAPHIC ANALYSIS OF THE INTERACTION OF THE GLUCOCORTICOID RECEPTOR WITH DNA
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 263 |
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.500, 95.700, 120.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.500 * - 2.900 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.400 |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 8 * | pH 6.00, VAPOR DIFFUSION, temperature 281.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 2 | NA CACODYLATE | ||
| 2 | 1 | 1 | MG SULFATE | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 1 | ZNCL2 | ||
| 7 | 1 | 2 | WATER | ||
| 8 | 1 | 2 | MPD/ETOH | ||
| 9 | 1 | 2 | NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.07 (mM) | duplex |
| 10 | 1 | reservoir | MPD | 8 (%) | can be replaced with ethanol |
| 2 | 1 | drop | 440-525 | 0.14 (mM) | |
| 3 | 1 | drop | 1.8 (mM) | ||
| 4 | 1 | drop | 25 (mM) | ||
| 5 | 1 | drop | sodium cacodylate | 18 (mM) | |
| 6 | 1 | drop | spermine | 1.8 (mM) | |
| 7 | 1 | drop | 0.002 (mM) | ||
| 8 | 1 | reservoir | 0.2 (M) | ||
| 9 | 1 | reservoir | sodium cacodylate | 24 (mM) |
