1GLQ

1.8 ANGSTROMS MOLECULAR STRUCTURE OF MOUSE LIVER CLASS PI GLUTATHIONE S-TRANSFERASE COMPLEXED WITH S-(P-NITROBENZYL)GLUTATHIONE AND OTHER INHIBITORS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	102.300, 77.700, 57.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.185
Rwork	0.185
RMSD bond length	0.012
RMSD bond angle	2.660
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.059 *
Total number of observations	82541 *
Number of reflections	22182 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	15 (mg/ml)
2	1	drop	MES	10 (mM)
3	1	drop	glutathione sulphonic acid	18 (mM)
4	1	drop	MES	50 (mM)
5	1	drop		0.02 (%)
6	1	drop	PEG6000	20 (%)
7	1	drop	imidazole	50 (mM)
8	1	reservoir	sodium/phosphate phosphate	0.3 (M)