1GLP

1.8 ANGSTROMS MOLECULAR STRUCTURE OF MOUSE LIVER CLASS PI GLUTATHIONE S-TRANSFERASE COMPLEXED WITH S-(P-NITROBENZYL)GLUTATHIONE AND OTHER INHIBITORS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	102.100, 77.600, 57.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.172
Rwork	0.172
RMSD bond length	0.013
RMSD bond angle	2.660
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	1.800 *	1.900 *
Rmerge	79.000 *
Total number of observations	102401 *
Number of reflections	28654 *
Completeness [%]		64 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	15 (mg/ml)
2	1	drop	MES	10 (mM)
3	1	drop	glutathione sulphonic acid	18 (mM)
4	1	drop	MES	50 (mM)
5	1	drop		0.02 (%)
6	1	drop	PEG6000	20 (%)
7	1	drop	imidazole	50 (mM)
8	1	reservoir	sodium/phosphate phosphate	0.3 (M)