1GKH

MUTANT K69H OF GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	285
Detector technology	IMAGE PLATE
Collection date	1994-01
Detector	RIGAKU
Spacegroup name	C 1 2 1
Unit cell lengths	76.660, 27.750, 41.790
Unit cell angles	90.00, 102.20, 90.00

Refinement procedure

Resolution	8.000 - 1.700
R-factor	0.217
Rwork	0.217
R-free	0.31200
Structure solution method	DIFFERENCE FOURIERS
RMSD bond length	0.017
RMSD bond angle	27.200 *
Data reduction software	R-AXIS (IIC)
Data scaling software	R-AXIS (II)
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.780
High resolution limit [Å]	1.700	1.700
Rmerge	0.042
Number of reflections	7813
<I/σ(I)>	11
Completeness [%]	81.4	58.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5		PROTEIN WAS CRYSTALLIZED FROM SOLUTIONS CONTAINING 10 MG/ML PROTEIN, 4 MM TRIS BUFFER (PH7.5), AND 16% PEG 4000 (W/V), EQUILIBRATED AGAINST 12% PEG 4000 BY VAPOR DIFFUSION AT ROOM TEMPERATURE., vapor diffusion

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	4 (mM)
3	1	drop	PEG4000	16 (%(w/v))
4	1	reservoir	PEG4000	12 (%(w/v))