Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Collection date | 1995-07-15 |
| Detector | ADSC AREA DETECTOR |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 118.700, 101.380, 111.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.630 - 1.560 |
| R-factor | 0.178 * |
| Rwork | 0.178 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jbc |
| RMSD bond length | 0.020 * |
| RMSD bond angle | 1.850 * |
| Data reduction software | ADSC |
| Data scaling software | DATAMAN |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.630 | 1.600 |
| High resolution limit [Å] | 1.564 | 1.560 |
| Rmerge | 0.035 | 0.249 |
| Total number of observations | 234173 * | |
| Number of reflections | 73179 | 2920 * |
| <I/σ(I)> | 16 | 2.1 |
| Completeness [%] | 77.0 | 46 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | HANGING DROP, 30MG/ML, 10+10 UL 1 ML WELL, TRIS/HCL PH 8.0, 25% |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | in water |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 3 | 1 | reservoir | ethanol | 25 (%(v/v)) |
