1GJU

Maltosyltransferase from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 41 2 2
Unit cell lengths	148.740, 148.740, 106.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.208
Rwork	0.208
R-free	0.26300
Structure solution method	MIR
RMSD bond length	0.012
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.420
High resolution limit [Å]	2.400	2.380
Rmerge	0.047	0.138
Total number of observations	198549 *
Number of reflections	47952
<I/σ(I)>	23.9	9.9
Completeness [%]	99.0	98
Redundancy	4.2	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.8	290 *	Burke, J., (2000) Acta Crystallog., D56, 1049. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	50 (mM)
2	1	drop		150 (mM)
3	1	drop	protein	18 (mg/ml)
4	1	reservoir	ammonium phosphate	0.35-0.40 (M)