1GJR
Ferredoxin-NADP+ Reductase complexed with NADP+ by COCRYSTALLIZATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | I 41 |
| Unit cell lengths | 151.690, 151.690, 35.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.700 - 2.100 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1que |
| RMSD bond length | 0.011 |
| RMSD bond angle | 27.309 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.100 | 0.380 |
| Number of reflections | 23930 | |
| <I/σ(I)> | 4.4 | 1.9 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 20 * | pH 5.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 27 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 3 | 1 | drop | NADP+ | 10 (mM) | |
| 4 | 1 | drop | beta-octylglucoside | 5 (%(w/v)) | unbuffered |
| 5 | 1 | reservoir | PEG6000 | 20 (%(w/v)) | |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |
