1GJM
Covalent attachment of an electroactive sulphydryl reagent in the active site of cytochrome P450cam
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.100, 61.100, 215.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cpp |
| RMSD bond length | 0.070 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.076 |
| Total number of observations | 78650 * |
| Number of reflections | 23420 |
| <I/σ(I)> | 14.6 |
| Completeness [%] | 95.0 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 18 * | SEE MAIN REFERENCE, pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.6-1.9 (M) | |
| 2 | 1 | reservoir | 100 (mM) | ||
| 3 | 1 | reservoir | HEPES | 100 (mM) | |
| 4 | 1 | drop | protein | 5 (mM) | |
| 5 | 1 | drop | phosphate | 40 (mM) | |
| 6 | 1 | drop | 100 (mM) | ||
| 7 | 1 | drop | dithiothreitol | 5 (mM) |
