1GIL

STRUCTURE OF ACTIVE CONFORMATIONS OF GIA1 AND THE MECHANISM OF GTP HYDROLYSIS

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	80.500, 80.500, 106.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.23 *
Rwork	0.222
R-free	0.31000 *
RMSD bond length	0.013
RMSD bond angle	1.588 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.041 *
Total number of observations	58697 *
Number of reflections	16167
Completeness [%]	97.2 *
Redundancy	3.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-30 (mg/ml)
2	1	drop		0.3 (mM)
3	1	drop		5 (mM)
4	1	drop		5 (mM)
5	1	drop	DTT	10 (mM)
6	1	drop	sodium acetate	200 (mM)
7	1	reservoir	ammonium sulfate	1.8-1.9 (M)