1GII
HUMAN CYCLIN DEPENDENT KINASE 2 COMPLEXED WITH THE CDK4 INHIBITOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 294 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-20 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.912, 72.427, 53.965 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.202 * |
| Rwork | 0.202 |
| R-free | 0.25900 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.767 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (98.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.048 | 0.157 |
| Total number of observations | 68987 * | |
| Number of reflections | 19273 | |
| <I/σ(I)> | 25.2 | |
| Completeness [%] | 94.7 | 82.4 |
| Redundancy | 3.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | 4 * | HEPES, sodium chloride, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES-NaOH | 10 (mM) | pH7.4 |
| 3 | 1 | drop | 15 (mM) | ||
| 4 | 1 | reservoir | HEPES-NaOH | 800 (mM) | pH7.4 |
