1GIG

REFINED THREE-DIMENSIONAL STRUCTURE OF THE FAB FRAGMENT OF A MURINE IGG1, LAMBDA ANTIBODY

Experimental procedure

Spacegroup name	P 31 2 1
Unit cell lengths	98.900, 98.900, 89.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 2.300
R-factor	0.195
RMSD bond length	0.012
RMSD bond angle	0.043
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.057 *	0.155 *
Total number of observations	68753 *
Number of reflections	21391 *
Completeness [%]	93.6 *	86.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		18 *	referred to J.Mol.Biol. 216.513-514 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HC19 Fab fragment	10 (mg/ml)
2	1	drop		0.15 (M)
3	1	drop		0.1 (%)
4	1	reservoir		1.2 (M)
5	1	reservoir		0.1 (%)