1GIF

HUMAN GLYCOSYLATION-INHIBITING FACTOR

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-04-25
Detector	RIGAKU
Spacegroup name	P 31 2 1
Unit cell lengths	96.600, 96.600, 105.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.168
Rwork	0.168
RMSD bond length	0.018
RMSD bond angle	28.410 *
Data reduction software	PROCESS
Data scaling software	SCALE
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Rmerge	0.072
Number of reflections	43864
Completeness [%]	93.0
Redundancy	2.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12.5 (mg/ml)
2	1	drop	HEPES	0.05 (M)
3	1	drop	ammonium sulfate	1.0 (M)
4	1	drop	PEG400	1 (%(w/v))
5	1	reservoir	HEPES	1.0 (M)
6	1	reservoir	ammonium sulfate	2.0 (M)
7	1	reservoir	PEG400	2 (%(w/v))