1GID
CRYSTAL STRUCTURE OF A GROUP I RIBOZYME DOMAIN: PRINCIPLES OF RNA PACKING
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1995-12-30 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.800, 128.700, 145.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.28500 |
| Structure solution method | MAD-SIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 13.800 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.860 |
| High resolution limit [Å] | 2.500 | 2.770 |
| Number of reflections | 36401 | |
| <I/σ(I)> | 23.7 | 4.7 |
| Completeness [%] | 73.0 | 80.2 |
| Redundancy | 4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | pH 6.00, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | K CACODYLATE | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | [CO(NH3)6]CL3 | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | potassium cacodylate | 60 (mM) | |
| 2 | 1 | reservoir | magnesium chloride | 30 (mM) | |
| 3 | 1 | reservoir | spermine | 0.3 (mM) | |
| 4 | 1 | reservoir | cobalt hexammine chloride | 0.2-1.0 (mM) | |
| 5 | 1 | reservoir | methylpentanediol |
