1GH6
RETINOBLASTOMA POCKET COMPLEXED WITH SV40 LARGE T ANTIGEN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-01 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 |
| Unit cell lengths | 127.130, 127.130, 96.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 3.200 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gux |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.440 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 3.290 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.084 | 0.369 * |
| Total number of observations | 50139 * | |
| Number of reflections | 12666 | |
| <I/σ(I)> | 15.9 | 2.8 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * | pH 7.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Rb pocket | 20 (mg/ml) | |
| 2 | 1 | drop | T antigen | 30 (mg/ml) | |
| 3 | 1 | drop | Tris-HCl | 25 (mM) | |
| 4 | 1 | drop | 150 (mM) | ||
| 5 | 1 | drop | dithiothreitol | 5 (mM) | pH7.5 |
| 6 | 1 | reservoir | PEG6000 | 4-6 (%) | |
| 7 | 1 | reservoir | potassium phosphate | 70 (mM) | |
| 8 | 1 | reservoir | dithiothreitol | 10 (mM) | pH7.2 |
