1GH0
CRYSTAL STRUCTURE OF C-PHYCOCYANIN FROM SPIRULINA PLATENSIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY |
| Synchrotron site | Photon Factory |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Detector | WEISSENBERG |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.000, 117.500, 185.000 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.189 * |
| Rwork | 0.189 |
| R-free | 0.23700 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 * | 0.313 * |
| Number of reflections | 296302 | 14756 * |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 95.4 * | 94.4 * |
| Redundancy | 2.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 | 298 | pH 6.8, vapour diffusion/hanging drop, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.5 (mg/ml) | |
| 2 | 1 | drop | phosphate | 100 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 8 (%(v/v)sat) | |
| 4 | 1 | drop | 3 (%(v/v)sat) | ||
| 5 | 1 | drop | MPD | 1.25 (%(v/v)) | |
| 6 | 1 | reservoir | ammonium sulfate | 25 (%(v/v)sat) | |
| 7 | 1 | reservoir | phosphate | 100 (mM) |
