1GGX

RED FLUORESCENT PROTEIN (FP583 OR DSRED(CLONTECH)) FROM DISCOSOMA SP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	56.206, 129.621, 57.441
Unit cell angles	90.00, 99.15, 90.00

Refinement procedure

Resolution	28.400 - 1.900
R-factor	0.192
Rwork	0.192
R-free	0.21200
Structure solution method	OTHER
RMSD bond length	0.007
RMSD bond angle	26.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.400	2.020
High resolution limit [Å]	1.900	1.900
Rmerge	0.086 *	0.401 *
Total number of observations	525892 *
Number of reflections	62456
<I/σ(I)>	14.5	2.5
Completeness [%]	97.9	79
Redundancy	3.6	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5	20 *	pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MPD	54 (%)
2	1	reservoir	Tris-HCl	0.1 (M)