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1GGX

RED FLUORESCENT PROTEIN (FP583 OR DSRED(CLONTECH)) FROM DISCOSOMA SP.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]100
Detector technologyIMAGE PLATE
DetectorRIGAKU RAXIS IV
Spacegroup nameP 1 21 1
Unit cell lengths56.206, 129.621, 57.441
Unit cell angles90.00, 99.15, 90.00
Refinement procedure
Resolution28.400 - 1.900
R-factor0.192
Rwork0.192
R-free0.21200
Structure solution methodOTHER
RMSD bond length0.007
RMSD bond angle26.100

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]28.4002.020
High resolution limit [Å]1.9001.900
Rmerge0.086

*

0.401

*

Total number of observations525892

*

Number of reflections62456
<I/σ(I)>14.52.5
Completeness [%]97.979
Redundancy3.62.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

8.520

*

pH 8.5
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirMPD54 (%)
21reservoirTris-HCl0.1 (M)

229380

PDB entries from 2024-12-25

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