1GGX
RED FLUORESCENT PROTEIN (FP583 OR DSRED(CLONTECH)) FROM DISCOSOMA SP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.206, 129.621, 57.441 |
Unit cell angles | 90.00, 99.15, 90.00 |
Refinement procedure
Resolution | 28.400 - 1.900 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.21200 |
Structure solution method | OTHER |
RMSD bond length | 0.007 |
RMSD bond angle | 26.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.400 | 2.020 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.086 * | 0.401 * |
Total number of observations | 525892 * | |
Number of reflections | 62456 | |
<I/σ(I)> | 14.5 | 2.5 |
Completeness [%] | 97.9 | 79 |
Redundancy | 3.6 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | 20 * | pH 8.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MPD | 54 (%) | |
2 | 1 | reservoir | Tris-HCl | 0.1 (M) |