1GGU

HUMAN FACTOR XIII WITH CALCIUM BOUND IN THE ION SITE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-06-01
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	100.172, 70.764, 133.822
Unit cell angles	90.00, 106.11, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.227
Rwork	0.227
R-free	0.31300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fie
RMSD bond length	0.017
RMSD bond angle	27.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.660	2.030
High resolution limit [Å]	2.000	2.100 *
Rmerge	0.043	0.669
Number of reflections	111498
Completeness [%]	93.0 *	86 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.2		pH 6.20

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	1,2-propanediol	24 (%)
2	1	1	sodium potassium phosphate	100 (mM)