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THREE-DIMENSIONAL STRUCTURE OF A TRANSGLUTAMINASE: HUMAN BLOOD COAGULATION FACTOR XIII
Experimental procedure
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 101.200, 182.700, 93.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.650 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.183 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 * | 20-22 * | Bishop, P.D., (1990) J.Biol.Chem., 23, 13888. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 20 (%sat) | 0.8M |
| 2 | 1 | drop | ammonium sulfate | 10 (%sat) | 0.4M |
| 3 | 1 | drop | sodium acetate | 20 (mM) | |
| 4 | 1 | drop | 2-mercaptoethanol | 10 (mM) | |
| 5 | 1 | drop | 1,2,3-heptanetriol | 0.2 (%) | |
| 6 | 1 | drop | factor XIII | 8.2 (mg/ml) |
