1GGO
T453A MUTANT OF PYRUVATE, PHOSPHATE DIKINASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-04-15 |
| Detector | SIEMENS |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 89.730, 58.660, 102.780 |
| Unit cell angles | 90.00, 95.58, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.29400 |
| Structure solution method | OTHER |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.076 | 0.236 |
| Total number of observations | 43302 * | |
| Number of reflections | 27567 | |
| <I/σ(I)> | 11.7 | 2.1 |
| Completeness [%] | 83.0 | 68 |
| Redundancy | 1.6 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 30 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 50-55 (%sat) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | drop | imidazole | 20 (mM) | |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | drop | EDTA | 0.1 (mM) | |
| 7 | 1 | drop | mercaptoethanol | 1 (mM) |
