1GGL

HUMAN CELLULAR RETINOL BINDING PROTEIN III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	59.514, 59.841, 68.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.990 - 2.310
Rwork	0.229
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1crb
RMSD bond length	0.011
RMSD bond angle	27.200 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.430
High resolution limit [Å]	2.300	2.300
Rmerge	0.127 *	0.332 *
Number of reflections	9874
<I/σ(I)>	11.2	3.8
Completeness [%]	88.0	76.5
Redundancy	3.7	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5	21 *	pH 5.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000
2	1	reservoir	potassium phosphate	25 (mM)