1GGK

CRYSTAL STRUCTURE OF CATALASE HPII FROM ESCHERICHIA COLI, ASN201HIS VARIANT.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-01-01
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	95.230, 134.700, 124.450
Unit cell angles	90.00, 109.41, 90.00

Refinement procedure

Resolution	117.380 - 2.260
R-factor	0.143
Rwork	0.130
R-free	0.20800
RMSD bond length	0.010
RMSD bond angle	0.031
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.540	2.320
High resolution limit [Å]	2.260	2.260
Rmerge	0.089 *	0.355 *
Number of reflections	120733	10095 *
Completeness [%]	88.2	68.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	297	PEG 3350, LiCl, Tris-HCl, pH 9.0, vapor diffusion/hanging drop, temperature 297.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG3350	15-17 (%)
3	1	reservoir		1.6-1.5 (M)
4	1	reservoir	Tris-HCl	0.1 (M)