1GGG

GLUTAMINE BINDING PROTEIN OPEN LIGAND-FREE STRUCTURE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1990-07-25
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	86.300, 86.300, 81.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.203
Rwork	0.203
R-free	0.28900
Structure solution method	ISIRAS
RMSD bond length	0.013
RMSD bond angle	1.500
Data reduction software	XENGEN (V. 2.0)
Data scaling software	XENGEN (V. 2.0)
Phasing software	ISIR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	2.460
High resolution limit [Å]	2.300	2.300
Rmerge	0.051	0.251
Total number of observations	100231 *
Number of reflections	24247
<I/σ(I)>	17.37	2.045
Completeness [%]	92.6	61
Redundancy	4.1	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.6	18 *	pH 8.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	0.1 (M)
3	1	drop	ammonium sulfate	50 (%sat)
4	1	reservoir	ammonium sulfate	75 (%sat)