1GGE

CRYSTAL STRUCTURE OF CATALASE HPII FROM ESCHERICHIA COLI, NATIVE STRUCTURE AT 1.9 A RESOLUTION.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1996-01-01
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	93.040, 132.340, 121.200
Unit cell angles	90.00, 109.63, 90.00

Refinement procedure

Resolution	18.000 * - 1.890
R-factor	0.166 *
Rwork	0.163
R-free	0.20200
RMSD bond length	0.008 *
RMSD bond angle	0.025 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.960	1.940
High resolution limit [Å]	1.890	1.890
Rmerge	0.089 *	0.269 *
Number of reflections	214389	16094 *
Completeness [%]	97.7	88.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	297	PEG 3350, LiCl, Tris-HCl, pH 9.00, VAPOR DIFFUSION, HANGING DROP, temperature 297.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG3350	15-17 (%)
3	1	reservoir		1.6-1.5 (M)
4	1	reservoir	Tris-HCl	0.1 (0.1)