1GGD
CRYSTAL STRUCTURE OF GAMMA CHYMOTRYPSIN WITH N-ACETYL-LEUCIL-PHENYLALANINE ALDEHYDE BOUND AT THE ACTIVE SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-11-29 |
| Detector | SIEMENS |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 69.600, 69.600, 97.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.500 |
| R-factor | 0.182 * |
| Rwork | 0.182 |
| R-free | 0.21200 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | XCALIBRE |
| Refinement software | X-PLOR (3.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.043 | 0.184 |
| Number of reflections | 38043 * | |
| Completeness [%] | 88.0 | 55.1 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | ammonium sulfate, potassium phosphate, pH 7.0, vapor diffusion, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 75 (%sat) | |
| 2 | 1 | reservoir | potassium phosphate | 10 (mM) |
