1GEW

CRYSTAL STRUCTURE OF HISTIDINOL-PHOSPHATE AMINOTRANSFERASE COMPLEXED WITH PYRIDOXAL 5'-PHOSPHATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1999-01-18
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	133.330, 63.820, 46.200
Unit cell angles	90.00, 104.07, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.2
Rwork	0.197
R-free	0.24700
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.417
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.069	0.230
Total number of observations	88073 *
Number of reflections	25542
<I/σ(I)>	12.7
Completeness [%]	96.7	85.6
Redundancy	2.7	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	20 *	PEG 4000, magnesium chloride, tris, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-25 (mg/ml)
2	1	reservoir	PEG4000	21 (%(v/v))
3	1	reservoir		200 (mM)
4	1	reservoir	Tris-HCl	100 (mM)