1GER

THE STRUCTURE OF GLUTATHIONE REDUCTASE FROM ESCHERICHIA COLI AT 1.86 ANGSTROMS RESOLUTION: COMPARISON WITH THE ENZYME FROM HUMAN ERYTHROCYTES

Experimental procedure

Spacegroup name	P 1 1 21
Unit cell lengths	120.500, 73.600, 60.500
Unit cell angles	90.00, 90.00, 83.00

Refinement procedure

Resolution	7.000 - 1.860
R-factor	0.168
Rwork	0.168
RMSD bond length	0.016
RMSD bond angle	2.830
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.860 *
Rmerge	0.091 *
Number of reflections	83086 *
Completeness [%]	95.1 *	78.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-30 (mg/ml)
2	1	drop	potassium phosphate	100 (mM)
3	1	drop	PEG8000	7 (%)
4	1	drop		0.02 (%)
5	1	reservoir	potassium phosphate	100 (mM)
6	1	reservoir	PEG8000	20 (%)