1GEH

CRYSTAL STRUCTURE OF ARCHAEAL RUBISCO (RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	FUJI
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	233.750, 233.750, 93.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	100.000 - 2.800
R-factor	0.224 *
Rwork	0.224
R-free	0.25400
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	1.404 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.091	0.335
Total number of observations	784332 *
Number of reflections	64453
<I/σ(I)>	11.8
Completeness [%]	89.5	65.5
Redundancy	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9	20 *	Maeda, N., (1999) J.Mol.Biol., 293, 57. *
1	Vapor diffusion, hanging drop *	9	20 *	Maeda, N., (1999) J.Mol.Biol., 293, 57. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ammonium sulfate	1.8 (M)
3	1	drop		10 (mM)
4	1	drop	CHES-NaOH	100 (mM)	pH9.0