1GE8

PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) HOMOLOG FROM PYROCOCCUS FURIOSUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	296
Detector technology	CCD
Collection date	2000-05-18
Detector	ADSC QUAMTUM 4r
Spacegroup name	P 63
Unit cell lengths	89.682, 89.682, 63.269
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.530 - 2.100
R-factor	0.225 *
Rwork	0.225
R-free	0.25290
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.530	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.083	0.470
Total number of observations	121358 *
Number of reflections	19669
<I/σ(I)>	17.1
Completeness [%]	99.8	99.9
Redundancy	6.2	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	20 *	ammonium sulfate, 1,4-dioxane, sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	100 (mM)
2	1	reservoir	ammonium sulfate	2.4 (M)
3	1	reservoir	1,4-dioxane	5 (%(v/v))
4	1	drop	protein	20 (mg/ml)