1GE8
PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) HOMOLOG FROM PYROCOCCUS FURIOSUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 296 |
| Detector technology | CCD |
| Collection date | 2000-05-18 |
| Detector | ADSC QUAMTUM 4r |
| Spacegroup name | P 63 |
| Unit cell lengths | 89.682, 89.682, 63.269 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.530 - 2.100 |
| R-factor | 0.225 * |
| Rwork | 0.225 |
| R-free | 0.25290 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.530 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 | 0.470 |
| Total number of observations | 121358 * | |
| Number of reflections | 19669 | |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 20 * | ammonium sulfate, 1,4-dioxane, sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.4 (M) | |
| 3 | 1 | reservoir | 1,4-dioxane | 5 (%(v/v)) | |
| 4 | 1 | drop | protein | 20 (mg/ml) |
