1GE7

ZINC PEPTIDASE FROM GRIFOLA FRONDOSA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-11-02
Detector	RIGAKU RAXIS IV
Spacegroup name	P 43
Unit cell lengths	30.202, 30.202, 308.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.179 *
Rwork	0.179
R-free	0.21830
RMSD bond length	0.005
RMSD bond angle	1.080
Data reduction software	PROCESS
Data scaling software	PROCESS ((RIGAKU))
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.300	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.097	0.341
Total number of observations	137781 *
Number of reflections	18300 *
<I/σ(I)>	9.6
Completeness [%]	98.5	98.1
Redundancy	7.1	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5 *	293 *	sodium chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	acetate	10 (mM)
3	1	reservoir		0.1-3.0 (M)
4	1	reservoir	imidazole	10 (mM)