1GE7
ZINC PEPTIDASE FROM GRIFOLA FRONDOSA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-02 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 43 |
| Unit cell lengths | 30.202, 30.202, 308.049 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.179 * |
| Rwork | 0.179 |
| R-free | 0.21830 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.080 |
| Data reduction software | PROCESS |
| Data scaling software | PROCESS ((RIGAKU)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.300 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.097 | 0.341 |
| Total number of observations | 137781 * | |
| Number of reflections | 18300 * | |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 98.5 | 98.1 |
| Redundancy | 7.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 * | 293 * | sodium chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | acetate | 10 (mM) | |
| 3 | 1 | reservoir | 0.1-3.0 (M) | ||
| 4 | 1 | reservoir | imidazole | 10 (mM) |
