1GE6
ZINC PEPTIDASE FROM GRIFOLA FRONDOSA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-02 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 58.650, 58.650, 145.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.200 - 2.200 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.23300 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.120 |
| Data reduction software | PROCESS |
| Data scaling software | PROCESS ((RIGAKU)) |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.200 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.075 | 0.184 |
| Total number of observations | 50233 * | |
| Number of reflections | 8115 * | |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 6.1 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 * | 293 * | sodium chloride , pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | acetate | 10 (mM) | |
| 3 | 1 | reservoir | 0.1-3.0 (M) | ||
| 4 | 1 | reservoir | imidazole | 10 (mM) |
