1GE6

ZINC PEPTIDASE FROM GRIFOLA FRONDOSA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-11-02
Detector	RIGAKU RAXIS IV
Spacegroup name	P 65 2 2
Unit cell lengths	58.650, 58.650, 145.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.200 - 2.200
R-factor	0.198 *
Rwork	0.198
R-free	0.23300
RMSD bond length	0.006
RMSD bond angle	1.120
Data reduction software	PROCESS
Data scaling software	PROCESS ((RIGAKU))
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.200	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.075	0.184
Total number of observations	50233 *
Number of reflections	8115 *
<I/σ(I)>	13.2
Completeness [%]	99.5	99.7
Redundancy	6.1	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5 *	293 *	sodium chloride , pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	acetate	10 (mM)
3	1	reservoir		0.1-3.0 (M)
4	1	reservoir	imidazole	10 (mM)