1GE5

ZINC PEPTIDASE FROM GRIFOLA FRONDOSA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1994-12-15
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1
Unit cell lengths	30.800, 40.500, 30.500
Unit cell angles	105.90, 91.70, 103.50

Refinement procedure

Resolution	29.800 - 2.000
R-factor	0.184 *
Rwork	0.184
R-free	0.22200
RMSD bond length	0.006
RMSD bond angle	1.160
Data reduction software	PROCESS
Data scaling software	PROCESS ((RIGAKU))
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.800	3.000
High resolution limit [Å]	2.000	2.250
Rmerge	0.135	0.199
Total number of observations	17364 *
Number of reflections	6440
<I/σ(I)>	5.4
Completeness [%]	64.8	43.4
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5 *	293 *	sodium chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	acetate	10 (mM)
3	1	reservoir		0.1-3.0 (M)
4	1	reservoir	imidazole	10 (mM)