1GD9
CRYSTALL STRUCTURE OF PYROCOCCUS PROTEIN-A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-11 |
| Detector | ADSC QUAMTUM 4r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.670, 122.280, 127.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.21 * |
| Rwork | 0.210 |
| R-free | 0.24200 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.530 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.086 | 0.297 |
| Total number of observations | 272833 * | |
| Number of reflections | 83030 | |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 96.0 | 96 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | 1,6-hexane diol, Tris, magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 5 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | protein | 0.2 (mM) | |
| 4 | 1 | reservoir | 1,6-hexanediol | 3 (M) | |
| 5 | 1 | reservoir | HEPES | 100 (mM) | |
| 6 | 1 | reservoir | 10 (mM) |
