1GD8

THE CRYSTAL STRUCTURE OF BACTERIA-SPECIFIC L17 RIBOSOMAL PROTEIN.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-01-15
Detector	MAR CCD 165 mm
Spacegroup name	P 41
Unit cell lengths	109.239, 109.239, 128.841
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.213
Rwork	0.210
R-free	0.25400
RMSD bond length	0.008
RMSD bond angle	1.320
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.051	0.245
Number of reflections	63819
<I/σ(I)>	11.7
Completeness [%]	95.2	86.8
Redundancy	4.5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	303	PEG MME 5000, Lithium sulphate, Sodium chloride, Methanol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 303K