1GD2

CRYSTAL STRUCTURE OF BZIP TRANSCRIPTION FACTOR PAP1 BOUND TO DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-05-12
Detector	RIGAKU RAXIS IV++
Spacegroup name	H 3
Unit cell lengths	240.910, 240.910, 43.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.230
R-free	0.25300
RMSD bond length	0.006
RMSD bond angle	18.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.600	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.052	0.244
Number of reflections	53501
<I/σ(I)>	10.3
Completeness [%]	83.4	60.2
Redundancy	2.4	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.8	277	PEG 6000, KCl, MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MES
2	1	1	KCl
3	1	1	PEG 6000
4	1	2	PEG 6000