1GCZ
MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) COMPLEXED WITH INHIBITOR.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 100 |
Detector technology | DIFFRACTOMETER |
Collection date | 1999-05-25 |
Detector | WEISSENBERG |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.600, 67.400, 87.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.24000 |
Structure solution method | MR |
Starting model (for MR) | 1gd0 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | X-PLOR (98.1) |
Refinement software | X-PLOR (98.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.035 | 0.489 |
Number of reflections | 31955 | |
<I/σ(I)> | 8.6 | |
Completeness [%] | 85.5 | 71.5 |
Redundancy | 3.1 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | ammonium sulfate, sodium citrate, pH 5.0, vapor diffusion/hanging drop, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 100 (mg/ml) | |
2 | 1 | drop | beta-mercaptoethanol | 14.4 (mM) | |
3 | 1 | drop | PMSF | 0.1 (mM) | |
4 | 1 | drop | sodium citrate | 100 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 0.6-1.5 (M) | |
6 | 1 | reservoir | HEPES | 0.1 (M) |