1GCY

HIGH RESOLUTION CRYSTAL STRUCTURE OF MALTOTETRAOSE-FORMING EXO-AMYLASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-05-14
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	69.420, 171.660, 46.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.600
R-factor	0.259
Rwork	0.259
R-free	0.30400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2amg
RMSD bond length	0.006
RMSD bond angle	25.200 *
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
High resolution limit [Å]	1.600
Rmerge	0.061
Total number of observations	222219 *
Number of reflections	66492
Completeness [%]	89.6
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	4 *	ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25.7 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	ammonium sulfate	0.2 (M)