1GC7
CRYSTAL STRUCTURE OF THE RADIXIN FERM DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 288 |
| Detector technology | CCD |
| Collection date | 2000-03-02 |
| Detector | ADSC QUAMTUM 4r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 96.330, 96.330, 133.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| Rwork | 0.215 |
| R-free | 0.25500 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.863 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.046 | 0.393 |
| Number of reflections | 23751 | |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 4 * | PEG 6000, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | MES-Na | 50 (mM) | |
| 3 | 1 | reservoir | PEG6000 | 2 (%) | |
| 4 | 1 | reservoir | 150 (mM) | ||
| 5 | 1 | reservoir | dithiothreitol | 0.5 (mM) |
