1GC5
CRYSTAL STRUCTURE OF A NOVEL ADP-DEPENDENT GLUCOKINASE FROM THERMOCOCCUS LITORALIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 286 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1999-01-01 |
| Detector | WEISSENBERG |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 109.800, 109.800, 129.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.300 |
| R-factor | 0.204 * |
| Rwork | 0.204 |
| R-free | 0.25200 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.414 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.043 | 0.177 |
| Total number of observations | 167633 * | |
| Number of reflections | 46477 | |
| <I/σ(I)> | 18.6 | |
| Completeness [%] | 77.0 | 45.8 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 25 * | potassium chloride, citrate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | 1.8-2.0 (M) | ||
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 4 | 1 | reservoir | ADP | 2 (mM) | |
| 5 | 1 | reservoir | 10 (mM) |
