1GC2
CRYSTAL STRUCTURE OF THE PYRIDOXAL-5'-PHOSPHATE DEPENDENT L-METHIONINE GAMMA-LYASE FROM PSEUDOMONAS PUTIDA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 133.576, 133.576, 213.675 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.430 - 2.000 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.24300 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.260 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.079 |
| Total number of observations | 1016993 * |
| Number of reflections | 130168 |
| <I/σ(I)> | 7.9 |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 298 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 298 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 298 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 15 (%) | |
| 2 | 1 | reservoir | 250 (mM) | ||
| 3 | 1 | reservoir | MES-NaOH | 200 (mM) | pH6.5 |
| 4 | 1 | reservoir | PLP | 0.5 (mM) | |
| 5 | 1 | drop | 2-mercaptoethanol | 0.5 (%) | |
| 6 | 1 | drop | protein | 10-20 (mg/ml) | |
| 7 | 1 | drop | sodium phosphate | 20 (mM) | pH7.2 |
| 8 | 1 | drop | PLP | 0.5 (mM) | |
| 9 | 1 | drop | 2-mercaptoethanol | 0.5 (%) |
