1GC2
CRYSTAL STRUCTURE OF THE PYRIDOXAL-5'-PHOSPHATE DEPENDENT L-METHIONINE GAMMA-LYASE FROM PSEUDOMONAS PUTIDA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 133.576, 133.576, 213.675 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.430 - 2.000 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.24300 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.260 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.079 |
Total number of observations | 1016993 * |
Number of reflections | 130168 |
<I/σ(I)> | 7.9 |
Completeness [%] | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 298 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 298 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 298 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 15 (%) | |
2 | 1 | reservoir | 250 (mM) | ||
3 | 1 | reservoir | MES-NaOH | 200 (mM) | pH6.5 |
4 | 1 | reservoir | PLP | 0.5 (mM) | |
5 | 1 | drop | 2-mercaptoethanol | 0.5 (%) | |
6 | 1 | drop | protein | 10-20 (mg/ml) | |
7 | 1 | drop | sodium phosphate | 20 (mM) | pH7.2 |
8 | 1 | drop | PLP | 0.5 (mM) | |
9 | 1 | drop | 2-mercaptoethanol | 0.5 (%) |