1GC0

CRYSTAL STRUCTURE OF THE PYRIDOXAL-5'-PHOSPHATE DEPENDENT L-METHIONINE GAMMA-LYASE FROM PSEUDOMONAS PUTIDA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 1
Unit cell lengths	72.861, 81.030, 81.282
Unit cell angles	70.56, 63.17, 63.38

Refinement procedure

Resolution	71.460 - 1.700
R-factor	0.211 *
Rwork	0.210
R-free	0.23800 *
RMSD bond length	0.005
RMSD bond angle	1.237 *
Refinement software	CNS (0.9)

Data quality characteristics

	Overall
High resolution limit [Å]	1.700
Rmerge	0.037
Number of reflections	144898
<I/σ(I)>	28.5
Completeness [%]	90.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2 *	298	pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	15 (%)
2	1	reservoir	ammonium sulfate	250 (mM)
3	1	reservoir	Tris-HCl	200 (mM)	pH8.5
4	1	reservoir	PLP	0.5 (mM)
5	1	reservoir	2-mercaptoethanol	0.5 (%)
6	1	drop	protein	10-20 (mg/ml)
7	1	drop	sodium phosphate	20 (mM)	pH7.2
8	1	drop	PLP	0.5 (mM)
9	1	drop	2-mercaptoethanol	0.5 (%)