1GAW

CRYSTAL STRUCTURE ANALYSIS OF THE FERREDOXIN-NADP+ REDUCTASE FROM MAIZE LEAF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	288
Detector technology	DIFFRACTOMETER
Collection date	1999-07-07
Detector	WEISSENBERG
Spacegroup name	P 1 21 1
Unit cell lengths	74.680, 108.890, 44.370
Unit cell angles	90.00, 96.92, 90.00

Refinement procedure

Resolution	35.090 - 2.200
R-factor	0.194
Rwork	0.194
R-free	0.23700
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.340
High resolution limit [Å]	2.200 *	2.200
Rmerge	0.033 *	0.092 *
Total number of observations	67831 *
Number of reflections	29086 *
Completeness [%]	81.3 *	69.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	4 *	Ammonium Succinate, MES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	PEG4000	20 (%(w/v))
3	1	reservoir		100 (mM)
4	1	reservoir	Tris-HCl	50 (mM)